- 中文名称
Nitric Oxide (NO) Enzymatic Assay Kit, BioAssay
- 英文名字
- Nitric Oxide (NO) Enzymatic Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- N2577-02
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Analyte:This kit employs immunoaffinity purified Nitrate Reductase to allow you to measure total nitric oxide (NO) produced in in vitro experimental systems following enzymatic conversion to nitrite. Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem is the enzymatic conversion of nitrate to nitrite by the enzyme nitrate reductase (NaR), followed by quantitation of nitrite using Griess Reagent. In addition to providing all necessary components in a microtiter format, this kit employs affinity purified nitrate reductase and NADH, thereby circumventing some of the potential problems reported for nitric oxide measurement using NADPH dependent nitrate reductases.Principle of the Assay:In aqueous solution, nitric oxide rapidly degrades to nitrate and nitrite. Spectrophotometric quantitation of nitrite using Griess Reagent is straightforward, but does not measure nitrate. This kit employs the NADH-dependent enzyme nitrate reductase (NaR) for quantitative conversion of nitrate to nitrite prior to quantitation of nitrate using Griess reagent — thus providing for accurate determination of total NO production.This kit can be used to accurately measure as little as 1pmol/ul (~1uM) NO produced in aqueous solutions. Very little sample is required (5 to 85ul depending on the [NO] in the sample). The completed reaction is read at 540nm.Kit Components:*N2577-02A: Nitrate Reductase, 1x1ml N2577-02B: Nitrate Reductase Buffer, 1x1.5mlN2577-02C: Buffer, 1x25ml **N2577-02D: NADH, 1x2mg-Light Sensitive.N2577-02E: Color Reagent #1, 1x7ml N2577-02F: Color Reagent #2, 1x7ml-Light Sensitive.N2577-02G: Nitrate Standard, 1x1.5ml N2577-02H: Microtiter Plate, 1x 96 wellsStorage and Stability:*Store N2577-02A at -20°C. **Store N2577-02D powder at RT, liquid at -20°C; Light Sensitive. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Nitric Oxide (NO) Enzymatic Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Nitric Oxide (NO) Enzymatic Assay Kit, BioAssay产品后将其置于RT/4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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