- 中文名称
Nitric Oxide Assay Kit, BioAssay
- 英文名字
- Nitric Oxide Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- N2576-97
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules.Simple, direct and automation-ready procedures for measuring NO are becoming popular in Research and Drug Discovery. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2-/NO3- as a measure for NO level. Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite using improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 30 min.Key Features:Sensitive and accurate. Detection range 0.6-200uM in 96-well plate.Rapid and reliable. Using an optimized VCl3 reagent, the time required for reduction of NO3- to NO2- is 10 min at 60°C.Simple and high-throughput. The procedure involves mixing sample with three reagents, incubation for 10 min at 60°C and reading the optical density. Can be readily automated to measure thousands of samples per day.Applications:Direct Assays: NO in plasma, serum, urine, tissue/cells and foods.Drug Discovery/Pharmacology: effects of drugs on NO metabolism.Kit Contents: (100 tests in 96-well plates)Reagent A: 12ml Reagent B: 500ul Reagent C: 12mlNaOH: 1ml ZnSO4: 1ml Standard: 1mlStorage conditions. The kit is shipped at room temperature. Store all reagents at 2-8°C. Shelf life of six months after receipt.Precautions: reagents are for research use only. Please refer to Material Safety Data Sheet for detailed information.Procedures:Sample treatment: tissue or cell samples are homogenized in 1 x PBS (pH 7.4). Centrifuge at 10,000g or higher at 4°C. Use supernatant for NO assay. Samples that need deproteination include serum, plasma, whole blood, cell culture media containing FBS, tissue or cell lysates. Urine and saliva do not need deproteination.Deproteination. Mix 150ul sample with 8ul ZnSO4 in 1.5-mL tubes. Vortex and then add 8ul NaOH, votex again and centrifuge 10 min at 14,000 rpm. Transfer 100ul of the clear supernatant to a clean tube.Note: If samples need to be deproteinated, 150ul of each standard should be prepared and also treated with ZnSO4 and NaOH to eliminate the need for a dilution factor. Procedure using 96-well plate:1. Standards. Prepare 500ul 100uM Premix by mixing 50ul 1.0mM Standard and 450ul distilled water. Dilute standards in 1.5-mL centrifuge tubes as described in the Table.No Premix+H2O Nitrite (uM)1 250ul+0ul 1002 150ul+100ul 603 75ul+175ul 304 0ul+250ul 02. Reaction. Add 100ul of each sample to separate, labeled eppendorf tubes. (We recommend that samples be measured in at least duplicate). Immediately prior to starting the reaction, prepare enough Working Reagent (WR) for all samples and standards by mixing per reaction tube: 100ul Reagent A, 4ul Reagent B and 100ul Reagent C. Add 200ul of the WR to each sample and standard tube and incubate for 10 min at 60°C. (Alternatively, the reaction can be run at 37°C for 60 min or RT for 150 min.)3. Measurement. Briefly centrifuge the reaction tubes to pellet any condensation and transfer 250ul of each reaction to separate wells in a 96 well plate. Read OD at 500-570nm (peak 540 nm).Procedure using Cuvette:Prepare standards and samples as described for the 96-well procedure except quadruple (4×) the volumes. After the reaction, transfer 1ml to a cuvette. Measure OD540nm in the cuvette.Calculation:Subtract blank OD (Std 4) from the standard OD values and plot the OD against standard concentrations. Determine the slope using linear regression fitting. The NO concentration of Sample is calculated as[Nitric Oxide]=ODSAMPLE-ODBLANKSlope (uM)ODSAMPLE and ODBLANK are optical density values of the sample and water, respectively.Conversions: 1 mg/dL NO equals 333uM, 0.001% or 10 ppm.Materials Required, But Not Provided:Pipetting devices, eppendorf tubes, eppendorf centrifuge, clear, flat bottomed 96 well plates or cuvettes, plate reader or spectrophotometer and heat block or hot water bath (optional).General Considerations:Antioxidants and nucleophiles (e.g. b-mercaptoethanol, glutathione, dithiothreitol and cysteine) may interfere with this assay. Avoid using these compounds during sample preparation.
- 产品特点
- 针对Nitric Oxide Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Nitric Oxide Assay Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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