- 中文名称
Neopterin, Human, BioAssay ELISA Kit
- 英文名字
- Neopterin, Human, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- N1060-49
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Neopterin [D-erythro-neopterin] belongs to the group of pteridines. Neopterin as well as other pteridines are derived in vivo from guanosine-triphophate (GTP). Neopterin represents a precursor molecule of biopterin that is an essential cofactor in neurotransmitter synthesis, and it is also involved in variety of oxidation-reduction reactions in the body. The enzyme GTP-cyclohydrolase-I catalyzes this reaction in monocytes and macrophages. Neopterin is exclusively released by activated macrophages, and can be determined in serum, plasma and other body fluids. Neopterin biosynthesis is closely associated with activation of the cellular immune system. Higher levels of neopterin were found in patients with viral infections. Antigenic stimulation of human peripheral blood mononuclear cells leads to neopterin release into cell culture medium. Gamma Interferon treatment of macrophages in vitro also increases neopterin levels and generates highly reactive oxygen species (ROS). Neopterin is capable of enhancing ONOO- - as well as Cu(II)-mediated LDL oxidation, whereas 7,8-dihydroneopterin mainly protects LDL from oxidation.Changes in neopterin concentrations in serum or urine can predict complications such as graft rejection in organ transplant recipients. Elevated neopterin levels are found in autoimmune disorders such as rheumatoid arthritis and systemic lupus erythematosus (SLE). Neopterin levels can be used as prognostic predictors for certain types of malignancies. Measurement of neopterin levels has particular value for monitoring patients infected with HIV. Neopterin is eliminated primarily in the urine, so evaluation of urinary neopterin levels may be useful in assessing activation of the cellular immunity system even in the absence of typical clinical symptoms, since a correlation has been observed with the course of diseases involving cellular immunity activation and urinary levels.Principle of the Test:Neopterin ELISA kit is based on competitive binding of human Neopterin from serum samples and enzyme-labeled Neopterin to Neopterin specific antibodies immobilized on microtiter plates. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (blue color is inversely proportional to the amount of Neopterin present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow. Absorbance is then measured on an ELISA reader at 450nm and the concentration of Neopterin in samples and control is read off the standard curve.Range:Intra-assay Precision CV: < 10% for Neopterin range 2-50nmol/LInter-assay Precision CV: < 10% for Neopterin range 2-50nmol/LSensitivity:0.7nmol/LKit Components:N1060-49A: Micrototer Plate, 1x96 wellsN1060-49B: Standard 0nM, 1x1.5mlN1060-49C: Standard 1.35nM, 1x1.5mlN1060-49D: Standard 4nM, 1x1.5mlN1060-49E: Standard 12nM, 1x1.5mlN1060-49F: Standard 37nM, 1x1.5mlN1060-49G: Standard 111nM, 1x1.5mlN1060-49H: Assay Buffer, 1x21mlN1060-49J: Control 1, 4-8.3nM, 1x1.5mlN1060-49K: Control 2, 15.2-282nM, 1x1.5mlN1060-49L: Neopterin (HRP Conjugate, 1x13mlN1060-49M: Neopterin Antibody, 1x5mlN1060-49N: Wash Buffer, 20X, 1x50mlN1060-49P: TMB Substrate Solution, 1x19mlN1060-49Q: Stop Solution, 1N H2SO4, 1x19mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Neopterin, Human, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Neopterin, Human, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
