Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NAD+/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450nm.Kit Components:NADP/NADPH Extraction Buffer 1x50mlNADP Cycling Buffer 1x15mlNADP Cycling Enzyme Mix 1x1vialNADPH Developer 1x1vialStop Solution 1x1.2mlNADPH Standard (MW:833.36) 1x166.7ug.Storage and Stability:Store the kit at -70°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
