Assay of nicotinamide nucleotides is of continual interest in the studies of energy transforming and redox state of cells or tissues. The NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio. The NAD Cycling Enzyme Mix in the kit specifically recognizes NADH/NAD in an enzyme cycling reaction. There is no requirement to purify NADH/NAD from samples. The reaction specifically detects NADH and NAD, but not NADP nor NADPH. The enzyme cycling reaction significantly increases the detection sensitivity and specificity. NADt (NAD and NADH) or NADH can be easily quantified by comparing with standard NADH.Kit Components:NADH/NAD Extraction Buffer 1x50mlNAD Cycling Buffer 1x15mlNAD Cycling Enzyme Mix 1x1vialNADH Developer 1x1vialStop Solution 1x1.2mlNADH Standard (MW:763) 1x152.6ug.Storage and Stability:Store the kit at -70°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
