- 中文名称
NAD/NADH Assay Kit (Nicotinamide Adenine Dinucleotide), BioAssay, Colorimetric/Fluorometric
- 英文名字
- NAD/NADH Assay Kit (Nicotinamide Adenine Dinucleotide), BioAssay, Colorimetric/Fluorometric
- 供应商
- USBiological
- 产品货号
- N0009-19
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lex/em=530/585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.Applications:Direct Assays: NAD+/NADH concentrations and ratios in cell or tissue extracts.Key Features:Sensitive and Accurate: Detection limit of 0.02uM and linearity up to 1uM NAD+/NADH in 96-well plate assay.Convenient: The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min.High-throughput: Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.Kit Components:N0009-19A: Assay Buffer, 1x10ml N0009-19B: Lactate, 1x1.5mlN0009-19C: Probe, 1x750ulN0009-19D: Enzyme A, 1x120ulN0009-19E: Enzyme B, 1x120ulN0009-19F: NAD Standard, 1x500ulN0009-19G: NAD Extraction Buffer, 1x12mlN0009-19H: NADH Extraction Buffer, 1x12mlStorage and Stability:Store all reagents at -20°C. Avoid freeze/thaw cycles. Stable for 6 months after receipt.Precautions:Reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents.General Considerations:1. At these concentrations, the standard curves for NAD and NADH are identical. Since NADH in solution is unstable, we provide only NAD as the standard.2. This assay is based on an enzyme-catalyzed kinetic reaction. Addition of Working Reagent should be quick and mixing should be brief but thorough. Use of multi-channel pipettor is recommended.3. The following substances interfere and should be avoided in sample preparation. EDTA (>0.5mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%).4. For samples containing higher than 100uM pyruvate, we recommend using an internal standard.
- 产品特点
- 针对NAD/NADH Assay Kit (Nicotinamide Adenine Dinucleotide), BioAssay, Colorimetric/Fluorometric该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到NAD/NADH Assay Kit (Nicotinamide Adenine Dinucleotide), BioAssay, Colorimetric/Fluorometric产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
