我们的二酰甘油（DAG）检测试剂盒通过偶联酶促反应系统测量细胞裂解液样本中的二酰甘油。首先，使用激酶将样本中的二酰甘油磷酸化，生成磷脂酸。然后，使用脂肪酶将磷脂酸水解为甘油-3-磷酸。接着，甘油-3-磷酸产物被甘油-3-磷酸氧化酶氧化，产生过氧化氢。该反应释放的过氧化氢与试剂盒的荧光探针特异性反应，并在激发波长530-560纳米/发射波长585-595纳米处进行检测。未知样本中的二酰甘油水平根据所提供的二酰甘油标准曲线来确定。 Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.
