我们的总磷脂酸检测试剂盒通过偶联酶促反应系统测量细胞裂解液样本中的总磷脂酸含量（磷脂酸（PA）和溶血磷脂酸（LPA））。首先，使用脂肪酶将磷脂酸水解为甘油-3-磷酸。接着，甘油-3-磷酸产物被甘油-3-磷酸氧化酶（GPO）氧化，产生过氧化氢。该反应释放的过氧化氢与试剂盒的荧光探针特异性反应，并在激发波长530-560纳米/发射波长585-595纳米处进行检测。未知样本中的磷脂酸水平根据所提供的磷脂酸标准曲线来确定。 Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.
