Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Assay: 0.1M Acetate buffer, pH 4.5 0.1M p-Nitrophenyl glucuronide Enzyme solution in distilled water 0.5 N NaOH.