【干冰】2X SDS-PAGE Sample Buffer without DTT or b-ME (125 mM Tris, 20% Glycerol, 4% SDS, 0.01% bromophenol blue, pH 6.8) - MB-018-【干冰】2X SDS-PAGE Sample Buffer without DTT or b-ME (125 mM Tris, 20% Glycerol, 4% SDS, 0.01% bromophenol blue, pH 6.8)

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中文名称: 【干冰】2X SDS-PAGE Sample Buffer without DTT or b-ME (125 mM Tris, 20% Glycerol, 4% SDS, 0.01% bromophenol blue, pH 6.8) - MB-018

英文名字: 【干冰】2X SDS-PAGE Sample Buffer without DTT or b-ME (125 mM Tris, 20% Glycerol, 4% SDS, 0.01% bromophenol blue, pH 6.8) - MB-018

背景资料: SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins. Visit our newly expanded web site at www.rockland-inc.com for methods using this and other buffers.

产品描述: 2X SDS-PAGE Sample Loading Buffer is suitable for laboratory assays involved in protein biochemistry. 2X SDS-PAGE Sample Buffer is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample Buffer consists of 0.125 M Tris, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromophenol blue. This product is not reduced and does not contain DTT or b-ME. The pH of this solution is 6.8. It was meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.

保存建议: Store container at -20° C or colder prior to opening. Aliquot and re-freeze for best results. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility of some salts.

其他: Rockland成立于1962年，是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。