- 中文名称
Myoglobin, Human (MB, Parvalbumin, PVALB) BioAssay ELISA Kit
- 英文名字
- Myoglobin, Human (MB, Parvalbumin, PVALB) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- M9800-37
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Sample Type:SerumIntended Use:Myoglobin ELISA is intended for the quantitative determination of myoglobin in human serum.Introduction:Myoglobin, a heme protein with a molecular weight of approximately 17,500D is found in both cardiac and skeletal muscle. Damage to either type of muscle following conditions such as trauma, ischemia, and diseases that cause myopathy, is associated with the release of myoglobin into serum (1,2). Specifically, following cardiac necrosis associated with myocardial infarction (MI), myoglobin is one of the first markers to rise above normal levels. Myoglobin levels increase measurably above baseline within 2-4 hours post-infarct, peaking at 9-12 hours, and returning to baseline within 24-36 hours (1,3,4,5). In the absence of skeletal muscle trauma or other factors associated with a non-cardiac related increase in circulating myoglobin, its levels have been used as an early marker for myocardial infarct (4,6,7). Unlike the other cardiac enzymes such as creeatine kinase and the MB isoform (i.e., CK and CK/MB) which do not reach serum levels until several hours post-infarction (approx. 19 hours), myoglobin levels can be expected to peak within 6 to 9 hours (16). Myoglobin Enzyme Immunoassay provides a rapid, sensitive, and reliable assay for the quantitative measurement of myoglobin in serum. The antibodies developed for the test will determine a minimal concentration of 5ng/ml, and there is no cross-reactivity with related cardiac or skeletal enzymes.Test Principle:The Myoglobin ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay (17,18). The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the myoglobin molecule. Mouse monoclonal anti-myoglobin antibody is used for solid phase immobilization (on the microtiter wells). A goat anti-myoglobin antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the two antibodies, resulting in the myoglobin molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 45 minute incubation at room temperature, the wells are washed with water to remove unbound labeled antibodies. A TMB (Tetramethyl-benzidine) Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of myoglobin is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.Kit Components:Antibody-Coated Wells (1 plate, 96 wells), Microtiter wells coated with murine monoclonal anti-myoglobin.Standard, 0ng/ml, 1x1mlStandard, 25ng/ml, 1x1mlStandard, 100ng/ml, 1x1mlStandard, 250ng/ml, 1x1mlStandard, 500ng/ml, 1x1mlStandard, 1000ng/ml, 1x1mlSample Diluent (25ml/bottle), Contains phosphate buffer and 1% (w/v) Pro-Clin as preservative.Enzyme Conjugate Reagent (22ml/vial), Contains anti-myoglobin conjugated to horseradish peroxidase in Tris buffer-BSA solution with preservatives.TMB Reagent (11ml/bottle), Contains one-step TMB solution.Stop Solution (1 bottle, 11 ml/bottle), Contains diluted hydrochloric acid (1N HCl).Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Myoglobin, Human (MB, Parvalbumin, PVALB) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Myoglobin, Human (MB, Parvalbumin, PVALB) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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