- 中文名称
Mycoplasma pneumonia, IgM, BioAssay ELISA Kit
- 英文名字
- Mycoplasma pneumonia, IgM, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- M9750-17
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The United States Biological Inc. Mycoplasma IgM kit is a Enzyme-Linked Immunosorbent Assay (ELISA) intended for the qualitative determination of IgM antibodies in human serum to Mycoplasma pneumoniae antigen. The anti- Mycoplasma IgM assay may be used as an aid in the diagnosis of Mycoplasma pneumoniae infection in the adult population. High complexity test.Mycoplasma pneumoniae is among the smallest of free-living organisms. In humans, it is usually found in the throat and lungs and is susceptible to antimicrobial agents that inhibit protein synthesis (1). The spread of mycoplasmal infections depends on close and prolonged contact between people, as it is transmitted by means of droplet nuclei from the respiratory tract (2). Mycoplasmal infection may be asymptomatic or may produce upper respiratory tract disease or atypical pneumonia. The pneumonia is difficult to differentiate from viral diseases by clinical means alone (1,2). Laboratory tests such as isolation, Complement Fixation serology, and ELISA serology are helpful as an aid in the diagnosis (1, 2, 3, 4).Principle of the Assay:Enzyme-linked immunosorbent assays (ELISA) rely on the ability of biological materials, (i.e. antigens) to adsorb to plastic surfaces such as polystyrene (solid phase). When antigens bound to the solid phase are brought into contact with a patient's serum, antigen specific antibody, if present, will bind to the antigen on the solid phase forming antigen- antibody complexes. Excess antibody is removed by washing. This is followed by the addition of goat anti-human IgM globulin conjugated with horserumdish peroxidase which then binds to the antibody-antigen complexes. The excess conjugate is removed by washing, followed by the addition of substrate and chromogen, Tetramethylbenzidine (TMB). If specific antibody to the antigen is present in the patient's serum, a blue color develops. When the enzymatic reaction is stopped with 1N H2SO4, the contents of the wells turn yellow. The color, which is proportional to the concentration of antibody in the serum, can be read on a suitable spectrophotometer or ELISA microwell plate reader (5, 6, 7, 8). The sensitivity, specificity, and reproducibility of enzyme-linked immnuoassays can be comparable to other serological tests for antibody, such as immunofluorescence, complement fixation, hemagglutination and radioimmunoassays (9, 10, 11).Detection Range Qualitative: Positive, Negative and Cut-offSample: 10ulSpecificity: 93%Sensitivity: 100%Total Time: 60minShelf Life: 12-18monthsKit Components: Each kit contains the following components in sufficient quantities to perform the number of tests indicated. All reactive reagents contain 0.1% sodium azide as a preservative .1. Microtiter Plate2. Conjugate3. Calibrator4. Positive Control5. Negative Control6. TMB7. Wash Buffer, 10X Diluted wash buffer to 1X8. Sample Diluent9. Stop Solution Storage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Mycoplasma pneumonia, IgM, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Mycoplasma pneumonia, IgM, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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