- 中文名称
Immobilized Mismatch Binding Protein (IMBP) BioGenomics Kit
- 英文名字
- Immobilized Mismatch Binding Protein (IMBP) BioGenomics Kit
- 供应商
- USBiological
- 产品货号
- M9557-01B
- 产品报价
- ￥23
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Immobilized Mismatch Binding Protein (IMBP) 96-well Plates are a breakthrough in mutation/polymorphism detection. IMBP plates are coated microtiter plates designed to bind DNA molecules containing single base pair mismatches or small (1-4 base) additions or deletions. IMBP plates are suited for single nucleotide polymorphism (SNP) detection, allele identification, mutation scanning, PCR optimization and PCR fidelity monitoring. Typical IMBP assays require less than one hour to complete and do not require gel electrophoresis or radioactivity. IMBP plates are available as individual plates (12 strips of 8 wells) or in kits containing plates, buffers, DNA standards and colorimetric reagents. IMBP 96-well plates provide a robust, economical and easy-to-use assay to detect, identify and even isolate sequences which contain any type of single base alteration. Kit Components:IMBP Plate, control oligos, hybridization and reaction buffers and colormetric detection reagents.Note:For Kit, order 1KitFor Plate only, order 1PlateSimple Protocol:IMBP plate assays are simple to perform. After DNA amplification and hybridization, results are obtained in less than 60 minutes using standard laboratory equipment and supplies.1. Rinse to remove storage buffer2. Add DNA to wells. Incubate 15-30 minutes3. Wash off unbound DNA4. Add Streptavidin-HRP. Incubate 15 minutes5. Wash6. Incubate with TMB7. Add stop acid and measure OD (450 nm)Scanning:Samples can be screened for SNPs by simply amplifying with biotin labeled primers and denaturing and annealing the PCR product to allow heterozygous SNPs to form mismatches with wild type DNA. See Examples of SNP detection in fragments of BRCA1 and p53 genes.SNP Enrichment:IMBP plates can be used to enrich SNP and other polymorphisms from mixed samples containing normal and tumor cells or pooled samples.PCR Fidelity Optimization:PCR fidelity is critical to many applications including mutation detection and cloning. PCR errors include both misincorporation and mispriming. Only IMBP assays detect both types of errors and can be used to measure PCR fidelity rapidly and inexpensively for any template.
- 产品特点
- 针对Immobilized Mismatch Binding Protein (IMBP) BioGenomics Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Immobilized Mismatch Binding Protein (IMBP) BioGenomics Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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