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细胞分析试剂盒
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中文名称

Mitochondria Isolation Kit, BioAssay, Tissue and Cultured Cells

英文名字
Mitochondria Isolation Kit, BioAssay, Tissue and Cultured Cells
供应商
USBiological
产品货号
M3986-10
产品报价
¥1/1Kit
产品说明书
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背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Mitochondria Isolation Kits are designed to isolating intact and active mitochondria in a fast and easy way. One kit is consisted of reagents enough for performing 100 isolations, enriching mitochondria from 10-20g tissue or from 1 to 5 x10e10 cells.Mitochondria are the eukaryotic subcellular organelles that contain the enzymes of the citric acid cycle, the electron transport chain, and oxidative phosphorylation. As new discoveries of human disorders are being related to mitochondrial dysfunction, there is an increasing need for an effective method to isolate intact mitochondria from tissues and cultured cells. The key steps when isolating mitochondria from any tissue or cell are always the same: (i) rupturing of cells by mechanical and/or chemical means and (ii) differential centrifugation at low speed to remove debris and extremely large cellular organelles followed by centrifugation at a higher speed to isolate mitochondria which are collected. The procedures described in this manual are easy and fast, and have been designed to provide the highest possible yield of intact and enzymatically active mitochondria.The isolated mitochondria can be used for numerous downstream applications, including apoptosis studies, metabolic studies and mitochondria diseases studies. This kit can also be used for isolating mitochondrial proteins for proteomics research, western blot and ELISA.Quality Control:Tested through the complete isolation procedure. Yield, Activity and Integrity of mitochondria isolated using this kit are comparable to those obtained with control from previous lots.Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对Mitochondria Isolation Kit, BioAssay, Tissue and Cultured Cells该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Mitochondria Isolation Kit, BioAssay, Tissue and Cultured Cells产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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