- 中文名称
Mitochondria, Human, BioAssay ELISA Kit, Primary Biliary Cirrhosis
- 英文名字
- Mitochondria, Human, BioAssay ELISA Kit, Primary Biliary Cirrhosis
- 供应商
- USBiological
- 产品货号
- M3986-01
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Systematic autoimmune disease is characterized by the presence of circulating autoantibodies directed to a wide variety of cellular antigens. Systemic lupus erythematosis (SLE), commonly referred to as Lupus is the best known of these diseases. Other possible connective tissue diseases include mixed connective tissue disease (MCTD), Sjogren syndrome, sclerodema, and polymyositis/dermatomyositis.Mitochondria (M2) antigen is part of the pyruvate dehydrogenase complex. Approximately 90-95% of patients with primary biliary cirrhosis (PBC) have antibodies to M2. Antimitochondria antibodies occur occasionally in other liver conditions and scleroderma. The antibody is rarely seen in other conditions. Classically, antibodies to autoimmune antigens are detected by double immunodiffusion. However, the test is lengthy and suffers weak sensitivity. ELISAs combine greater sensitivity with ease of use. Many ELISAs have been developed and validated for detecting autoantibodies to various antigens.Intended Use:Mitochondria, BioAssay ELISA Kit is intended for the detection and semi-quantitation of antibodies to mitochondria in patient serum. The assay is to be used to detect antibodies in a single serum specimen. The results of the assay are to be used as an aid to the diagnosis of primary biliary cirrhosis. Principle of the Assay:The United States Biological Mitochondria, BioAssay ELISA Kit is used to detect IgG, IgA, and IgM antibodies to Mitochondria antigens. Purified Mitochondria antigens are attached to a solid phase microassay well. Enzyme-Linked Immunosorbent Assays (ELISA) rely on the ability of biological materials (i.e., antigens) to adsorb to plastic surfaces such as polystyrene (solid phase). When antigens bound to the solid phase are brought into contact with a patient's serum, antigen specific antibody, if present, will bind to the antigen on the solid phase forming antigen-antibody complexes. Excess antibody is removed by washing. This is followed by he addition of an IgG, IgA, IgM (HRP) goat anti-human which then binds to the antibody-antigen complexes. The excess conjugate is removed by washing, followed by the addition of Chromogen/Substrate, tetramethylbenzidine (TMB). If specific antibody to the antigen is present in the sample, a blue color develops. When the enzymatic reaction is stopped with 1N H2SO4, the contents of the wells turn yellow. The color, which is indicative of the concentration of antibody in the serum, can be read on a suitable spectrophotometer or ELISA microwell plate reader. Kit Components:M3986-01A: Microtiter Plate: 1x96 wellsM3986-01B: Calibrator: ≥0.3 1x400ulM3986-01C: Negative Control 0.0-0.8: 1x400ulM3986-01D: Positive Control, High >1.4: 1x400ulM3986-01E: Positive Control, Low 1.0-2.1: 1x400ulM3986-01F: Serm Diluent: 1x30mlM3986-01G: IgG, IgA, IgM (HRP) Goat x human: 1x15mlM3986-01H: Wash Buffer, 20x: 1x50mlM3986-01J: Tetramethylbenzidine (TMB): 1x15mlM3986-01K: Stop Solution: 1x15mlStorage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Mitochondria, Human, BioAssay ELISA Kit, Primary Biliary Cirrhosis 该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Mitochondria, Human, BioAssay ELISA Kit, Primary Biliary Cirrhosis 产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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