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中文名称

b2-Microglobulin, Human (B2M, Beta 2 Microglobulin Precursor, Beta Chain of MHC Class 1 Proteins) BioAssay ELISA Kit

英文名字
b2-Microglobulin, Human (B2M, Beta 2 Microglobulin Precursor, Beta Chain of MHC Class 1 Proteins) BioAssay ELISA Kit
供应商
USBiological
产品货号
M3890-02N
产品报价
¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Human b-2 Microglobulin (B2MG) is an 11.8kD protein identical to the light chain of the HLA-A, -B, and –C antigen. B2MG is expressed on nucleated cells, and is found at low levels in the serum and urine of normal individuals. B2MG concentrations are increased in inflammatory diseases, some viral diseases, renal dysfunction, and autoimmune diseases. A number of publications are available which explain the interpretation of B2MG serum levels in assessing the status of individuals with various clinical conditions.Sample Type: SerumIntended Use: For the quantitative determination of B-2 Microglobulin (B2MG) concentration in human serum. Test Principle: The B2MG ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the intact b-2 Microglobulin molecule. Mouse monoclonal anti- B2MG antibody is used for solid phase immobilization (on the microtiter wells). A sheep anti-B2MG antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The diluted test sample is allowed to react first with the immobilized antibody for 30 minutes at 37°C. The sheep anti-B2MG-HRP conjugate is then added and reacted with the immobilized antigen for 30 minutes at 37°C, resulting in the B2MG molecules being sandwiched between the solid phase and enzymelinked antibodies. The wells are washed with water to remove unbound-labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes at room temperature, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution, changing the color to yellow. The concentration of B2MG is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.Kit Components:Microtiter wells 1x96 wells Murine monoclonal anti-B2MG antibody coatedStandard, 0, 0.625ug/ml, 1x1vialStandard, 1.25ug/ml, 1x1vialStandard, 2.5ug/ml, 1x1vialStandard, 5ug/ml, 1x1vial Standard, 10ug/ml, 1x1vial Sample Diluent, 1x100ml.Enzyme Conjugate Reagent, 1x22ml.TMB Reagent (One-Step), 1x11ml.Stop Solution (1N HCl), 1x11ml.Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
产品特点
针对b2-Microglobulin, Human (B2M, Beta 2 Microglobulin Precursor, Beta Chain of MHC Class 1 Proteins) BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到b2-Microglobulin, Human (B2M, Beta 2 Microglobulin Precursor, Beta Chain of MHC Class 1 Proteins) BioAssay ELISA Kit产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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