- 中文名称
Methionine Sulfoxide Western Blot, BioAssay Kit
- 英文名字
- Methionine Sulfoxide Western Blot, BioAssay Kit
- 供应商
- USBiological
- 产品货号
- M3022-01
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Several amino acid residues on proteins are modified upon reaction with reactive oxygen species (ROS). Although it has been extensively documented that methionine residues can be oxidized to methionine sulfoxide and, in contrast to some other adducts produced upon oxidation, methionine sulfoxide residues can be converted back to methionine by the action of methionine sulfoxide reductase. Thus, in addition to methionine sulfoxide residues serving as biomarkers for protein oxidation, this particular oxidation may play a role in redox signaling within the body.Intended Use:This kit has been tested for Western Blot. Other uses have not been tested.Kit Components:M3022-01A: Methionine Sulfoxide Pab Rabbit 1x100ulM3022-01B: Standard protein with extensive methionine sulfoxide modifications, 1x100ulM3022-01C: Methionine Sulfoxide 2mM solution used to suppress immunoreactivity, 1x100ulStorage and Stability:Store kit and its components at -20°C until use. Aliquot to avoid repeated freeze/thaw cycles. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Reagent Preparation:1. M3022-01A: Methionine Sulfoxide Pab Rabbit . The recommended starting dilution for Western Blot is 1:2000. 2. M3022-01B: Standard: Load 10ul/lane. The standard is a protein with extensive methionine sulfoxide modifications.3. M3022-01C: Methionine Sulfoxide: 2mM solution of free methionine sulfoxide that can be used to ensure specificity and to confirm reactivity by blocking all specific reactivity of the antibody. Incubate the antibody with the 2mM solution of methionine sulfoxide for one hour at 37°C then overnight at 4°C.Notes for Western Blot Procedure:The antibody and standard are compatible with common Western Blot procedures. For best results, the following is suggested:1. The nitrocellulose membrane should be blocked with PBS, 5% dry milk over night.2. The antibody should be diluted 1:2000 in PBS, 1% dry milk, 0.05% tween-20.3. Purified proteins being tested as targets for methionine oxidation should be treated with and without 200mM H2O2 overnight at RT. These samples should then be run on 4-20% SDS gel followed by common Western Blot procedures.4. Non-purified proteins from cells or tissue extracts should not be treated with H2O2, but protein enrichment may be required.
- 产品特点
- 针对Methionine Sulfoxide Western Blot, BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Methionine Sulfoxide Western Blot, BioAssay Kit产品后将其置于-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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