- 中文名称
Mesothelin, BioAssay ELISA Kit (Human) (CAK1 Antigen, Pre-pro-megakaryocyte-potentiating Factor, MSLN, MPF)
- 英文名字
- Mesothelin, BioAssay ELISA Kit (Human) (CAK1 Antigen, Pre-pro-megakaryocyte-potentiating Factor, MSLN, MPF)
- 供应商
- USBiological
- 产品货号
- M3006-02
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Mesothelin, also known as CAK1 and ERC, is a protein that is mainly expressed in the mesothelial cells lining the pleura, pericardium, and peritoneum. The Mesothelin gene encodes a precursor protein of 70kD that is cleaved into two products, including megakaryocyte potentiating factor (aa37-286) and Mesothelin (aa296-606). Megakaryocyte potentiation factor is a secreted protein of about 32kD, and it functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelin is a 40kD glycosylphosphatidylinositol-anchored cell-surface protein. Multiple transcription variants of Mesothelin, as a result of alternative splicing, have been described. Variant 1 is primarily expressed at the surface of the cells and can be shed. Compared to variant 1, variant 2 has an eight aa insertion between aa409-416 and variant 3 differs from aa601-630. Variant 1 is the predominant form that is expressed by both normal and tumor cells, while variants 2 and 3 exist at low levels. The biological functions of Mesothelin remain unclear. Bera et al. generated knockout mice in which the Mesothelin gene was inactivated. They found that these mice did not have any detectable abnormal phenotypes, and they produced offspring normally, suggesting that Mesothelin is not essential for growth and development. Other studies have reported that cells transfected with Mesothelin were more difficult to remove from the culture dishes than nontransfected cells. This indicates that Mesothelin might function as an adhesion molecule. This notion is further supported by the observation that Mesothelin is able to bind to CA125/MUC16 with a high affinity and that this interaction mediates cell adhesion. Mesothelin may thus play an important role in the peritoneal spread of ovarian cancer. The expression of Mesothelin is modulated by the Wnt proteins. In mouse mammary epithelial cells, Mesothelin is up-regulated by Wnt-1, while down-regulated by Wnt-5a. Under physiological conditions, the expression of Mesothelin is limited. However, overexpression of Mesothelin has been described in many types of malignancies, such as mesothelioma, ovarian cancer, and pancreatic cancer. Further studies have suggested that this cancer-specific overexpression of Mesothelin might be attributed to an upstream transcription enhancer. Mesothelin is detectable in many biological fluids, such as serum and urine. It has been demonstrated that quantification of Mesothelin in these biological fluids may serve as a useful biomarker for cancer diagnosis, prognosis, and monitoring response to therapy in patients with mesothelioma and ovarian cancer. Furthermore, due to its low expression in normal tissues and high expression in tumor cells, Mesothelin is an attractive candidate for cancer immunotherapy. Additionally, Mesothelin can elicit an autoimmune response in cancer patients. These therapies include agents that target cell surface Mesothelin or elicit an immune response against Mesothelin.Sample Type:Cell culture supernates, serum, plasma, saliva, and urineIntended Use:For the quantitative determination of human Mesothelin concentrations in cell culture supernates, serum, plasma, saliva, and urine.Sensitivity:Twenty assays were evaluated and the minimum detectable dose (MDD) of Mesothelin ranged from 0.005-0.022ng/ml. The mean MDD was 0.010ng/ml. The MDD was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.Specificity:Recognizes recombinant and natural human Mesothelin.Test Principle:This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mesothelin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Mesothelin present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for Mesothelin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Mesothelin bound in the initial step. The color development is stopped and the intensity of the color is measured.Kit Components:Mesothelin Microplate: 12 strips x 8 wellsMesothelin monoclonal antibody, conjugated to HRP: 1 x 21mlMesothelin Standard (lyophilized): 1 x 100ngAssay Diluent RD1S: 1 x 11mlCalibrator Diluent RD5-20 Concentrate: 1 x 21mlWash Buffer Concentrate: 1 x 21mlColor Reagent A (stabilized hydrogen peroxide): 1 x 12.5mlColor Reagent B (tetramethylbenzidine): 1 x 12.5mlStop Solution (2N sulfuric acid): 1 x 6mlPlate Covers: 4 adhesive stripsStorage and Stability:See Kit Protocol for detailed storage instructions.
- 产品特点
- 针对Mesothelin, BioAssay ELISA Kit (Human) (CAK1 Antigen, Pre-pro-megakaryocyte-potentiating Factor, MSLN, MPF)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Mesothelin, BioAssay ELISA Kit (Human) (CAK1 Antigen, Pre-pro-megakaryocyte-potentiating Factor, MSLN, MPF)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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