- 中文名称
MEK1, phosphorylated (Ser217/221) Sandwich ELISA Kit, BioAssay (Map Kinase Kinase, MAPK Kinase, Erk Kinase 1) (Human, Mouse)
- 英文名字
- MEK1, phosphorylated (Ser217/221) Sandwich ELISA Kit, BioAssay (Map Kinase Kinase, MAPK Kinase, Erk Kinase 1) (Human, Mouse)
- 供应商
- USBiological
- 产品货号
- M2865-02C
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Detects endogenous levels of phospho-MEK1 protein. A significant induction of MEK1 phosphorylation in PMA-treated NIH/3T3 cells can be detected using this kit. However, the level of total MEK1 protein detected by Total MEK1 Sandwich ELISA Kit remains unchanged This kit can also be used to detect phosphorylated MEK1 protein in human 293 cells.Phospho-MEK1 (Ser217/221) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Phospho-MEK1 (Ser217/221) protein. MEK1 Mouse mAb has been coated onto the microwells. After incubation with cell lysates, total MEK1 protein (phospho- and nonphospho-) is captured by the coated antibody. Following extensive washing, a Phospho-MEK1/2 Antibody is added to detect the captured phospho-MEK1 protein. HRP-linked anti-rabbit antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of phospho-MEK1 protein.MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1–3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221 (in the activation loop of subdomain VIII) by Raf-like molecules.MEK1/2 is activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (1–4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC12 cells (4). MEK activatesp44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.Products Volume Solution ColorMEK1 Mouse mAb coated Microwells 96 testsPhospho-MEK1/2 Detection Ab 11ml greenAnti-rabbit IgG HRP-Linked Ab 11ml redTMB Substrate 11ml colorlessSTOP Solution 11ml colorlessSealing Tape 2 sheets20X Wash Buffer 25ml colorlessSample Diluent 2 ml blue10X Cell Lysis Buffer15ml yellowishStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对MEK1, phosphorylated (Ser217/221) Sandwich ELISA Kit, BioAssay (Map Kinase Kinase, MAPK Kinase, Erk Kinase 1) (Human, Mouse)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到MEK1, phosphorylated (Ser217/221) Sandwich ELISA Kit, BioAssay (Map Kinase Kinase, MAPK Kinase, Erk Kinase 1) (Human, Mouse)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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