- 中文名称
MCAF, Human, BioAssay ELISpot Kit, Development Set (C-C Motif Chemokine 2, HC11, Monocyte Chemoattractant Protein 1, Monocyte Chemotactic and Activating Factor, MCP-1, Monocyte Secretory Protein JE, S
- 英文名字
- MCAF, Human, BioAssay ELISpot Kit, Development Set (C-C Motif Chemokine 2, HC11, Monocyte Chemoattractant Protein 1, Monocyte Chemotactic and Activating Factor, MCP-1, Monocyte Secretory Protein JE, Small-inducible Cytokine A2, CCL2, MCP1, SCYA2)
- 供应商
- USBiological
- 产品货号
- M2690-13M
- 产品报价
- ￥1/5x96Tests
- 产品说明书
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- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Intended Use:For the development of enzyme-linked immunospot (ELISpot) assays for the quantitation of single cells releasing human MCAF. For laboratory research use only. Not for use in diagnostic procedures.Background:Monocyte chemotactic and activating factor (MCAF), also known as monocyte chemotactic protein 1 (MCP-1), lymphocyte–derived chemotactic factor (LDCF), and glioma-derived chemotactic factor (GDF), is a recently-identified chemotactic cytokine for monocytes. CDNA cloning and structural analysis has revealed that this 76aa polypeptide with a predicted molecular mass of 8.7kD belongs to a family of structurally-related low molecular weight proteins characterised by four conserved cysteine residues designated C-C family or intercrine beta family (1,2,3).MCAF is expressed by various types of cultured cells including monocytes, lymphocytes, fibroblasts, endothelial cells, smooth muscle cells and transformed cell lines upon stimulation with LPS or cytokines such as IL-1, TNF-a, and IFN-g. Although there exist some minor differences in expression patterns that are observed in some types of cells, almost all agents that induce IL-8 mRNA expression also induce MCAF mRNA expression. Platelet-derived growth factor is a strong inducer of MCAF mRNA in human fibroblasts whereas it failed to induce IL-8 mRNA in human fibroblasts (4). These results suggest that the regulatory mechanism of MCAF gene expression differs from that of the IL-8 gene. In addition to being chemotactic for monocytes, MCAF also activates human monocytes to become cytostatic for several human tumour cell lines (5), release lysosomal enzymes (6), and generate superoxide (6).MCAF is also expressed in vivo by lung epithelial cells in patients with idiopathic pulmonary fibrosis (7), synovial tissues of rheumatoid arthritis (8), or in atheromatous plaques in atherosclerotic lesion (9), suggesting the participation of MCAF in the pathogenesis of these disorders. Furthermore, MCAF has a potent histamine-releasing activity on basophils (10) that indicates an associated effect in allergic inflammations. MCAF expression in vivo has been investigated by qualitative methods such as in situ hybridization and immunohistochemistry. In order to further clarify and elucidate its participation and relation with various disorders, quantitative analysis of its in vivo level is necessary (11, 12).Kit Compnents:Concentrated Human MCAF Capture Antibody: 1x1 vialConcentrated Human MCAF Detection Antibody: 1x1 vialConcentrated Streptavidin (AP): 1x1 vialStorage and Stability:Store at -20°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对MCAF, Human, BioAssay ELISpot Kit, Development Set (C-C Motif Chemokine 2, HC11, Monocyte Chemoattractant Protein 1, Monocyte Chemotactic and Activating Factor, MCP-1, Monocyte Secretory Protein JE, Small-inducible Cytokine A2, CCL2, MCP1, SCYA2)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到MCAF, Human, BioAssay ELISpot Kit, Development Set (C-C Motif Chemokine 2, HC11, Monocyte Chemoattractant Protein 1, Monocyte Chemotactic and Activating Factor, MCP-1, Monocyte Secretory Protein JE, Small-inducible Cytokine A2, CCL2, MCP1, SCYA2)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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