- 中文名称
Matrix Metalloproteinases, Colorimetric, BioAssay Kit
- 英文名字
- Matrix Metalloproteinases, Colorimetric, BioAssay Kit
- 供应商
- USBiological
- 产品货号
- M2420-08B
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Kit Size 100 Assays (96-well plate)This kit is optimized to detect MMP activity and screen for MMP inhibitors. It provides enough reagents to perform 100 assays in a 96-well format.High Speed: The entire process can be completed in minimal timeMatrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associatedzinc endopeptidases capable of digesting extracellular matrix components and processing bioactive mediators.1,2 Based on their role in normal and pathological processes, including embryogenesis, wound healing, inflammation, arthritis,3 and cancer,4,5 MMPs have been chosen as therapeutic targets for the treatment of many diseases.The MMP Assay Kit can be used for the continuous spectrophotometric assay of MMPs activity, including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14 and for high throughput screening of MMP inducers and inhibitors. The chromogenic substrate is a thiopeptolide that is cleaved by the MMPs, releasing a sulfhydryl group. The sulfhydryl group reacts with Ellman’s Reagent (5,5’-dithiobis(2-nitrobenzoic acid). The final product of this reaction, 2-nitro-5-thiobenzoic acid (TNB), can be detected at 412nm using a microplate reader. The assays are performed in a convenient 96-well microplate format.Kit Components:Component A Microtiter Plate 1x100 wells MMP colorimetric substrate 10mM, 1x100ulComponent B Reference standard 10mM, 1x10ulComponent C Assay Buffer 1x20mlComponent D MMP inhibitor 2mM, 1x10ulComponent E Trypsin 1mg/ml, 1x100ulComponent F Trypsin inhibitor 10mg/ml, 1x100ulComponent G Stop Solution 1x5mlMaterials Required (but not provided):• MMPs source: Enzymes, validated in MMPs assays, can be ordered from United States Biological: MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, mMMP-9), MMP-10, MMP-12, MMP-13, and MMP-1.• 96-well microplate: Clear microplates provide better signal for absorbance reading.• Microplate reader: Capable of detecting absorbance at 412nm.Storage and Handling:• Store all kit components at -20°C.• Aliquot Components E and F as needed to avoid freeze-thaw cycles.• Store Component C at 4°C in the dark.• Component G can be stored at room temperature for convenience. Stable for 6 months. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
- 产品特点
- 针对Matrix Metalloproteinases, Colorimetric, BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Matrix Metalloproteinases, Colorimetric, BioAssay Kit产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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