- 中文名称
MAP Kinase Immunoprecipitation Kinase Assay Kit, BioAssay
- 英文名字
- MAP Kinase Immunoprecipitation Kinase Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- M2353-50
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The assay kit is designed to measure phosphotransferase activity in an immunocomplex formed between the MAP Kinase R2 antibody and MAP Kinase (p44mpk). This precipitated enzyme is used to phosphorylate a specific substrate, myelin basic protein (MBP). The phosphorylated substrate is then analyzed by western immunoblot using an antibody specific for phosphorylated MBP. The measurement of MAPK activity in most celllysates is not accurate, due to the phosphorylation of MBP by other kinases. This enzyme assay is rapid, convenient and specific for MAP Kinase Applications:Non-radioactive immunoprecipitation and measurment of MAP kinases Capacity:20 assays Components: 5X ADB, (20-145) Anti-MAP Kinase 1/2 agaroseAnti-Phospho-MBPMAPK Substrate CocktailMAPK Inhibitor CocktaiMg/ATP CocktailOther components required but not included as part of kit are:• Enzyme preparation or stimulated cell extract containing active MAP Kinase• Buffer A: 50mM Tris, pH7.5, 1mM EDTA, 1mM EGTA, 0.5mM Na3VO4, 0.1% 2-mercaptoethanol, 1% Triton X-100, 50mM sodium fluoride, 5mM sodium pyrophosphate, 10mM sodium •-glycerol phosphate, 0.1mM PMSF, 1ug/ml of aprotinin,pepstatin, leupeptin, and 1uM Microcystin• vortex mixer• 30°C shaking incubator• timer• variable volume (5-200ul) pipet + tips• Western Immunoblot reagents and materialsStorage and Stability:Stable for 1 year at-20°C from date of shipment.Note: Upon arrival, store Anti-MAP Kinase 1/2, agarose at 4°C.
- 产品特点
- 针对MAP Kinase Immunoprecipitation Kinase Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到MAP Kinase Immunoprecipitation Kinase Assay Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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