P44/42 MAPK (Erk1 and Erk2), SAPK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, and the differentiation and control of cellular responses to cytokines and stress. p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK and p38 MAPK are activated by inflammatory cytokines and a wide variety of cellular stresses. Activation of SAPK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* resulting in p38 activation.MAPK Family Antibody Sampler Kit contains 20ul of each primary antibody (p44/42 MAP Kinase, SAPK/JNK, p38 MAP Kinase) and 50ul of anti-rabbit IgG secondary antibody labeled with HRP.Kit Components:M2352-40A: p44/42 MAP Kinase Antibody 1x20ulM2352-40B: SAPK/JNK Antibody 1x20ulM2352-40C: p38 MAP Kinase Antibody 1x20ulM2352-40D: Rabbit ant-Human IgG, HRP, 1x50ulApplications: Suitable for use in Western Blot and ELISA. Other applications not tested.Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
产品特点
针对MAPK Family BioAssay Sample Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
保存建议
建议收到MAPK Family BioAssay Sample Kit产品后将其置于-20°C保存。
