L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others. This is an easy and sensitive assay to measure the L(-) Malate level in a variety of samples. In the assay, malate is specifically oxidized to generate a product which reacts with a substrate probe to generate color (lambda-max = 450nm). The assay can detect 1~35nmol of Malate in a 50ul sample with a detection sensitivity ~20uM.Kit Components:Malate *Assay Buffer 1x20mlMalate Enzyme Mix lyophilizedWST Substrate lyophilizedMalate Standard (10umol) lyophilizedStorage and Stability:Store the kit at -20°C, protect from light. Allow *Assay Buffer to warm to RT before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
