WB; Western Blotting (WB). A concentration of 0.25-0.50 µg/mL is recommended for WB. Human ERK1 has a predicted length of 379 residues and MW of 43 kDa. Human ERK2 has a predicted length of 360 residues and MW of 41 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Synthetic peptide of 11 residues (HTGFLTEYVAT) conjugated to KLH. This antibody reacts with the epitope containing phosphorylated threonine and tyrosine residues within the regulatory site of active ERK1 and ERK2 (Thr202/Tyr204 for ERK1 and Thr185/Tyr187 for ERK2).
