WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25-0.5 µg/mL is recommended for WB. Human laminin (subunit alpha-1) has a predicted length of 3,075 residues and a MW of 337 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect laminin in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.