WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Bovine Calbindin-D has a predicted length of 261 residues and a MW of 28 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect Calbindin-D in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
