WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0-4.0 µg/mL is recommended for WB. Rabbit TNNT3 (isoform TNT1) has a predicted length of 279 residues and a MW of 33 kDa. A concentration of 4.0-8.0 µg/mL is recommended to detect the protein in paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 4.0-8.0 µg/mL is recommended to detect the protein in frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.