WB; IC; WB: Western blotting: 1-3 µg/mL, antibody is suitable for reduced (with DTT or beta-mercaptoethanol) and non-reduced samples. Denatured but not reduced samples provides cleaner blot signals as demonstrated in our photographs.
Immunocytochemistry: 1-2 µg/mL. Antibody works on 4% formaldehyde-fixed cells. Note that cells require a permeabilization step, because the antibody detects a cytoplasmic epitope of TrkA.
Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide from the intracellular cytoplasmic domain of human TrkA (C-HGPDAKLLAGGE, aa: 604-615) has been used as the immunogen.
来源宿主
小鼠
反应性
反应种属:人 TrkA. Known to cross-react with TrkA from 大鼠, 小鼠. Expected to cross-react with other mammalian species based on peptide antigen sequence similarity.