WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25-0.5 µg/mL is recommended for WB. Human beta actin has a predicted length of 375 residues and the MW of the monomer is 42 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect beta actin in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A modified synthetic peptide (C-DDDIAALVIDNGSGK) corresponding to the N-terminus of beta Actin conjugated to KLH.