WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC), Immunocytochemistry (ICC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Vinculin (isoform 2, Metavinculin) has a predicted length of 1,134 residues and a MW of 124 kDa. A concentration of 2.0-4.0 µg/mL is recommended to detect the protein in formalin/acetone fixed frozen tissues. A concentration of 2.0 µg/mL is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.