WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-2.0 µg/mL is recommended for WB. Human myoglobin (precursor) has a predicted length of 154 residues and MW of 17 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect myoglobin in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.