WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human Cytokeratin 18 has a predicted length of 430 amino acids and MW of 48 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. A concentration of 0.5-2.0 µg/mL is recommended to detect the protein in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Human epidermal carcinoma A431 and MCF7 human breast cancer cell lines