WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0-4.0 µg/mL is recommended for WB. Human HSP60 has a predicted length of 573 residues and MW of 61 kDa. A concentration of 4.0-8.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
