- 中文名称
Luteinizing Hormone, Human (LH) BioAssay ELISA Kit
- 英文名字
- Luteinizing Hormone, Human (LH) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- L7500-01
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- hLH (human Luteinizing Hormone (hLH) is found in the anterior pituitary gland. It induces ovulation and regulates progesterone and estrogen production by the ovaries and testes. LH levels are measured to aid diagnosing the source of certain infertility problems and to ascertain the existence of a pituitary tumor. Intended Use:This human LH ELISA kit is to be used for the in vitro quantitative determination of human luteinizing hormone (LH) concentrations in serum.Principles of the Assay: This LH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre- coated with a monoclonal antibody specific for LH. Standards or samples are then added to the microtiter plate wells and LH if present, will bind to the antibody pre-coated on the wells. In order to quantitate the amount of LH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for LH are added to each well to “sandwich” the LH immobilized on the plate. The microtiter plate undergoes an incubation, then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LH and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm. In order to measure the concentration of LH in the sample, this Human LH ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus LH concentration (mIU/ml). The concentration of LH in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: <3mIU/mlRange: 3-240mIU/mlKit Components:L7500-01A: Microtiter Plate 1x96 wellsL7500-01B: LH (HRP) 1x12mlL7500-01C: Standard (240 mIU) 1x vialL7500-01D: Standard (120 mIU) 1x1vialL7500-01E: Standard (60 mIU) 1x1vialL7500-01F: Standard (30 mIU) 1x1vialL7500-01G: Standard (7.5 mIU) 1x1vialL7500-01H: Standard (0 mIU) 1x1vialL7500-01J:. Substrate A 1x10mlL7500-01K: Substrate B 1x10mlL7500-01L: Stop Solution 1x7mlStorage and Stability:Store at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Luteinizing Hormone, Human (LH) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Luteinizing Hormone, Human (LH) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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