- 中文名称
Lipoprotein, High Density (HDL) and Lipoprotein, Low Density (LDL)/Lipoprotein, Very Low Density (VLDL) BioAssay Kit, Colorimetric/Fluorometric
- 英文名字
- Lipoprotein, High Density (HDL) and Lipoprotein, Low Density (LDL)/Lipoprotein, Very Low Density (VLDL) BioAssay Kit, Colorimetric/Fluorometric
- 供应商
- USBiological
- 产品货号
- L2600-57A
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors.Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex=585/530nm is directly proportional to total cholesterol concentration in the sample.Applications:Direct Assays: HDL and LDL/VLDL cholesterol in serum samples.Pharmacology: evaluation of drugs on cholesterol metabolism.Key Features:Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorometric assays.Convenient. Room temperature assay. No 37°C heater is needed.Kit Contents: (100 assays in 96-well plates)PBS: 2 × 1.5ml Precipitation Reagent: 1.5mlAssay Buffer: 20ml Enzyme Mix: 120uLDye Reagent: 120ul Standard: 1ml 300mg/dL cholesterolStorage conditions. Store PBS and Precipitation Reagent at room temperature and other reagents at -20°C. Shelf life: 6 months after receipt. Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Colorimetric Procedures:Important: bring all reagents except enzyme mix to room temperature prior to assay. Non-hemolyzed serum samples should be used.1. Sample Preparation. Transfer 20ul serum into a 1.5-mL centrifuge tube, add 20ul Precipitation Reagent. Vortex to mix and centrifuge 5 min at 9,500 x g (e.g. 9,500 rpm in an Eppendorf 5415C tabletop centrifuge).Carefully transfer 24ul supernatant into a clean tube, add 96ul Assay Buffer. Label this tube “HDL”.Carefully remove all remaining supernatant from the pellet. Transfer 40ul PBS to the pellet and mix by repeated pipetting. Transfer 24ul mixture into another clean tube, add 96ul Assay Buffer. Label this tube “LDL/VLDL”.In a third tube, transfer 12ul serum sample and mix well with 108ul Assay Buffer. Label this tube “Total”. Cholesterol Standard: transfer 5ul 300 mg/dL cholesterol and mix with 145ul Assay Buffer. Label this tube “Standard”.2. Assay. Transfer 50ul Assay Buffer (“Blank”), 50ul Standard, 50ul “Total”, 50ul “HDL” and 50ul “LDL/VLDL” into wells of a clear flat-bottom 96-well plate. If desired, run assays in duplicate.For each reaction well, mix 55ul Assay Buffer with 1ul Enzyme Mix and 1ul Dye Reagent. Add 50ul of this Working Reagent to each standard and sample well. Tap plate to mix well.Incubate 30 min at room temperature. Read OD values at 570 nm.Note: if the Sample OD is higher than the Standard OD, dilute sample inassay buffer and repeat the assay. Multiply result by the dilution factor.3. Calculation:. Cholesterol concentrations in the Total, HDL and (LDL/VLDL) fractions are calculated as follows,[Total] =ODTOTAL–ODBLANKODSTANDARD–ODBLANK× 100 (mg/dL)[HDL] =ODHDL–ODBLANKODSTANDARD–ODBLANK× 100 (mg/dL)[LDL/VLDL] =ODLDL/VLDL–ODBLANKODSTANDARD–ODBLANK× 100 (mg/dL)Fluorometric Procedure:Dilute the Samples and Standard prepared in Colorimetric Procedure: 1:10 in Assay Buffer. Transfer 50ul diluted standards and 50ul diluted samples into separate wells of a black 96-well plate.Add 50ul Working Reagent (see Colorimetric Procedure:). Tap plate to mix. Incubate 30 min at room temperature and read fluorescence at lex=530nm and lem=585nm.Note: if the Sample F is higher than the Standard F, dilute sample in assay buffer and repeat the assay. Multiply result by the dilution factor. The cholesterol concentration of Sample is calculated as[Total] =FTOTAL–FBLANKFSTANDARD–FBLANK× 100 (mg/dL)[HDL] =FHDL–FBLANKFSTANDARD–FBLANK× 100 (mg/dL)[LDL/VLDL] =FLDL/VLDL–FBLANKFSTANDARD–FBLANK× 100 (mg/dL)Materials Required, But Not Provided:Pipetting devices, 96-well plate and plate reader.Examples:Serum samples were run in duplicate according to the standard procedure.
- 产品特点
- 针对Lipoprotein, High Density (HDL) and Lipoprotein, Low Density (LDL)/Lipoprotein, Very Low Density (VLDL) BioAssay Kit, Colorimetric/Fluorometric该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Lipoprotein, High Density (HDL) and Lipoprotein, Low Density (LDL)/Lipoprotein, Very Low Density (VLDL) BioAssay Kit, Colorimetric/Fluorometric产品后将其置于RT/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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