- 中文名称
Lipid Peroxidation, Colorimetric Assay Kit, BioAssay
- 英文名字
- Lipid Peroxidation, Colorimetric Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- L2496-30
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides are unstable and decompose to form a complex series of compounds including reactive carbonyl compounds. Polyunsaturated fatty acid peroxides generate malondialdehyde (MDA) and 4-hydroxyalkenals (HAE) upon decomposition. The measurement of MDA has been used as an indicator of lipid peroxidation. This kit is designed to measure MDA.Assay Principle:This assay is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole (R1), with MDA at 45°C. One molecule of MDA reacts with 2 molecules of reagent R1 to yield a stable chromophore with maximal absorbance at 586nm.Kit Components:L2496-30A: Reagent R1 ( N-methyl-2-phenylindole in acetonitrile), 3 x18mlL2496-30B: MDA Standard (1,1,3,3-Tetramethoxypropane in Tris-HCl ), 1x1mlL2496-30C: Diluent (Ferric Iron in Methanol), 1x30mlStorage and Stability:Store kit components at 4°C. Kit is stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Materials Required But Not Supplied:1. Spectrophotometer for measuring absorbance at 586nm2. Spectrophotometric cuvettes with a 1cm optical path length3. Water bath at 45°C.4. Disposable glass test tubes and stoppers compatible with acetonitrile, methanol and acid.5. 37% HCl6. Butylated hydroxytoluene (BHT)7. Acetonitrile8. Microcentrifuge9. Polypropylene microcentrifuge tubes10. Deionized water11. Adjustable micropipettes (10-100ul) and tipsAssay Procedure:1. Add 200ul of Standards or Samples to a microcentrifuge tube.2. Add 650ul of diluted Reagent R1 to each tube and vortex.3. Add 150ul 37% (12N HCl) to each tube and mix well.4. Incubate at 45°C for 60 minutes.5. Centrifuge samples at 15,000 x g for 10 minutes to obtain a clear supernatant.6. Transfer the supernatant to a cuvette and read the absorbance at 596nm.
- 产品特点
- 针对Lipid Peroxidation, Colorimetric Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Lipid Peroxidation, Colorimetric Assay Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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