- 中文名称
Lipase Assay Kit, BioAssay
- 英文名字
- Lipase Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- L2496-08
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Lipase catalyzes the hydrolysis of ester bonds on the glycerol backbone of a lipid substrate. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglycerides to monoglycerides and free fatty acids. Human pancreatic lipase and its related protein 2 are the main lipases secreted by the pancreas. In acute pancreatitis, lipase levels can rise 5 to 10-fold within 24 to 48 hours. Increased activities have also been associated with pancreatic duct obstruction, pancreatic cancer, kidney disease, salivary gland inflammation, bowel obstruction, and other pancreatic diseases. Decreased levels may indicate permanent damage to lipase-producing cells in the pancreas.Simple, direct and automation-ready procedures for measuring lipase activity are very desirable. Lipase Assay is based on an improved dimercaptopropanol tributyrate (BALB) method, in which SH groups formed from lipase cleavage of BALB react with 5,5’-dithiobis(2-nitrobenzoic acid) (DTNB) to form a yellow colored product. The color intensity, measured at 412 nm, is proportionate to the enzyme activity in the sample.Applications:Direct assays of lipase activity in serum, plasma, saliva, urine and other biological samples.Key Features:Sensitive and accurate. Linear detection range 40 to 1600 U/L lipase activity in 96-well plate assay.Convenient and high throughput. The procedure involves adding a single working reagent, and reading the optical density at 10 min and 20 min at room temperature or 37°C. Can be automated to process thousands of samples per day.Kit Contents: (100 tests in 96-well plate)Assay Buffer (pH 8.5): 15ml Color Reagent: 530 mg BALB Reagent: 1.0ml Calibrator: 2.0ml (equivalent to 735 U/L)Storage conditions. This product is shipped at room temperature. Store all components at 4°C. Shelf life: 12 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Sample Preparation. Lipase inhibitors (EDTA, and certain detergents Tween-20, NP-40), mercaptoethanol and dithiothreitol interfere with this assay and should be avoided in sample preparation. Samples can be stored frozen for at least one month, if not assayed immediately. Tissue and cell lysates can be obtained by homogenization in cold PBS buffer and centrifugation (e.g. 5 min at 14,000 rpm).Preparation of Working Reagent. Mix Color Reagent into Assay Buffer and shake vial to mix. Add 0.8ml BALB Reagent (sufficient for 100 assays). Alternatively for partial reconstitution: for each well of reaction, mix 5 mg Color Reagent, 140ul Assay Buffer and 8ul BALB Reagent. The Working Reagent should be prepared freshly and used within one hour.Important: this assay is based on a kinetic reaction, addition of the Working Reagent should be quick. Use of a multi-channel pipettor is recommended.1. Transfer 150ul H2O and 150ul Calibrator into wells of a clear- bottom 96-well plate. Pipette 10ul samples into separate wells. Add 140ul Working Reagent to each sample well. Tap plate briefly to mix reaction mixture. Note: if the assay is to be performed at another temperature (e.g. 37°C), warm up the Working Reagent to this temperature prior to adding to the sample.2. Read OD412nm on a plate reader at 10 min (OD10min) and at 20 min (OD20min).3. Calculation:: lipase activity is calculated as follows, Activity=OD20min–OD10minx 735(U/L)where OD20min and OD10min are the OD412nm values of the sample at 20 min and 10 min, respectively. ODCalibrator and ODH2O are the OD412nm values of the Calibrator and water at 20 min. The number “735” is the equivalent activity (U/L) of the calibrator under the assay conditions.Note: if the calculated activity is higher than 1600 U/L, dilute sample in water and repeat assay. Multiply the results by the dilution factor (n).Unit definition: one unit of enzyme catalyzes the cleavage of 1uMole of substrate per minute under the assay conditions (pH 8.5).Materials Required, But Not Provided:Pipeting (multi-channel) devices. Clear-bottom 96-well plates (e.g. Corning Costar) and plate reader.For Assays In Cuvette:For assays in standard 1ml cuvet, use 1ml H2O and 1ml Calibrator. Reaction volumes: 60ul sample+940ul Working Reagent.Examples:Samples were assayed in duplicate using the 96-well plate protocol. Lipase activities were 809 ± 15 U/L for mouse serum, 959 ± 23 U/L for rat plasma, 665 ± 14 U/L for rat serum, 44 ± 1 U/L for goat serum, 80 ± 1 U/L for bovine serum, 75 ± 6 U/L for human serum and 52 ± 2 U/L for a human plasma sample.
- 产品特点
- 针对Lipase Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Lipase Assay Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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