- 中文名称
Leptin, Serum, Human, BioAssay ELISA Kit (Ob, Obese Protein, Obesity Factor)
- 英文名字
- Leptin, Serum, Human, BioAssay ELISA Kit (Ob, Obese Protein, Obesity Factor)
- 供应商
- USBiological
- 产品货号
- L1670-14L
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Recently, Ob genes (mouse and human) have been cloned. Obese gene encodes an adipocyte-tissue derived secreted Ob protein/Leptin (167aa, ~16kD) that controls body weight homeostasis. Exogenous administration of recombinant Ob protein can reduce food intake and body weight. However, Ob protein had no effect in db/db mice suggesting a defect in leptin signaling mechanism. Leptin exert its effects by interacting with leptin receptors (obese receptors). The Ob-R has been shown to be a product of db gene that has long been thought to encode the receptor for a weight-controlling hormone. The Ob-R has at least 6 alternatively spliced forms with modifications at the amino and C-terminus.Human Leptin ELISA kit is based on binding of Human Leptin from samples to two antibodies, one immobilized on the microtiter well plates, and other bound to the enhancing protein Biotin, which then binds to strepavidin horseradish peroxidase conjugate. After a washing step, chromogenic substrate is added and colors developed. The enzymatic reaction (color) is directly proportional to the amount of Leptin present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of Leptin in samples and control is read off the standard curve.Kit Components:L1670-14L1: Microwell Strip Plate, 1x96wellsL1670-14L2: Standard, 0ng/ml, 1x10mlL1670-14L3: Standard, 1ng/ml, 1x500ulL1670-14L4: Standard, 5ng/ml, 1x500ulL1670-14L5: Standard, 10ng/ml, 1x500ulL1670-14L6: Standard, 20ng/ml, 1x500ulL1670-14L7: Standard, 50ng/ml, 1x500ulL1670-14L8: Standard, 100ng/ml, 1x500ulL1670-14L9: Low Control, 1x500ulL1670-14L10: High Control, 1x500ulL1670-14L11: AB (Biotin), 1x10mlL1670-14L12: Streptavidin (HRP) 50X, 1x400ulL1670-14L13: Assay Buffer, 1x20mlL1670-14L14: Wash Buffer, 10X, 1x50mlL1670-14L15: TMB, 1x16mlL1670-14L16: Stop Solution, 1x6mlStorage and Stability:Store all components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Leptin, Serum, Human, BioAssay ELISA Kit (Ob, Obese Protein, Obesity Factor)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Leptin, Serum, Human, BioAssay ELISA Kit (Ob, Obese Protein, Obesity Factor)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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