- 中文名称
Leptin, Human (Ob, Obese Protein, Obesity Factor) BioAssay ELISA Kit
- 英文名字
- Leptin, Human (Ob, Obese Protein, Obesity Factor) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- L1670-14
- 产品报价
- ￥23
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- This Human Leptin ELISA kit is to be used for the in vitro quantitative determination of leptin in human serum, plasma, buffered solution or cell culture medium. The assay recognizes both natural and recombinant forms of leptin. Leptin, the product of the ob gene, is a hormone secreted by adipocytes. Animals with mutations in the ob gene are obese, diabetic and have reduced activity. Administration of recombinant leptin to these animals decreases food intake and causes weight loss. In humans, this type of mutation has not been found. Human leptin cDNA encodes a 167 amino acid non-glycosylated protein including a 21aa signal peptide which is cleaved to give mature human leptin. The human receptor for leptin (OB- B-R) has been identified as a 1144 amino acid transmembrane glycoprotein. t is expressed in the choroid plexus and in the hypothalamus. Leptin is implicated in an increasing number of endocrine regulations including adiposity, satiety, energy homeostasis, puberty and fertility. Little is known about the physiologic actions of leptin in humans. The availability of a sensitive and specific assay to measure concentrations of leptin in serum and plasma will accelerate our understanding.Principle:This Human Leptin kit is a solid phase sandwich ELISA. A monoclonal antibody specific for human leptin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known leptin content, control specimens, and unknowns, are pipetted into these wells followed by addition of a second biotinylated monoclonal antibody. During the first incubation, the leptin antigen binds to the immobilized (capture) antibody on one site and to the solution phase biotinylated antibody on a second site. After removal of excess secondary antibody, Streptavidin-Peroxidase enzyme is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of leptin present in the original specimen.Sensitivity: <3.5pg/mlRange: 15.6-1000pg/mlIncubation Time: 3 hours at RTSample size: 100ulAccuracy: Inter-assay CV: <5.3%Intra-assay CV: <3.9%Kit Components: 96 Test Kit192 Test KitL1670-14A Standard 2 vials 4 vialsL1670-14B Standard Diluent 1x60ml2x60mlL1670-14C Microtiter Plate1x96wells2x96wellsL1670-14D Mab (Biotin)1x11ml2x11mlL1670-14E Streptavidin (HRP), 100X1x125ul2x125ulL1670-14F HRP Diluent1x25ml1x25mlL1670-14G Wash Buffer, 25X1x100ml1x100mlL1670-14H TMB 1x25ml1x25mlL1670-14J Stop Solution1x25ml1x25mlStorage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Leptin, Human (Ob, Obese Protein, Obesity Factor) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Leptin, Human (Ob, Obese Protein, Obesity Factor) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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