- 中文名称
Legionella, IgG, IgA, IgM, BioAssay ELISA Kit
- 英文名字
- Legionella, IgG, IgA, IgM, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- L1663-11
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The United States Biological Legionella ELISA Test System is an enzyme-linked immunosorbent assay for the qualitative detection of total antibody (IgG/IgM/IgA) to Legionella pneumophila serogroups (1-6) in human serum. L. pneumophila was identified as the causative agent for Legionellosis (Legionella pneumonia, or Legionnaire's Disease) in 1977 (1). Presently, there are more than 25 species and 33 serogroups in the family Legionellaceae, with at least 18 species associated with pneumonia, accounting for roughly 1-5% of all cases of pneumonia (2). L. pneumophila displays a multitude of morphologies including the bacillus, coccobacillus, and elongated fusiform. Although often difficult to perform, the Gram stain will be Gram-negative. The Legionella IgG/IgA/IgM ELISA test system is designed to detect antibodies to L. pneumophila in human serum. Wells of plastic microwell strips are sensitized by passive absorption with Legionella antigen. The test procedure involves three incubation steps; 1. Test serum (properly diluted) are incubated in antigen coated microwells. Any antigen specific antibody in the sample will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components. 2. Peroxidase Conjugated goat anti-human IgG/IgA/IgM is added to the wells and the plate is incubated. The Conjugate will react with the antibody immobilized on the solid phase in step 1. The wells are washed to remove unreacted Conjugate. 3. The microwells containing immobilized peroxidase Conjugate are incubated with peroxidase Substrate Solution. Hydrolysis of the Substrate by peroxidase produces a color change. After a period of time the reaction is stopped and the color intensity of the solution is measured photometrically. The color intensity of the solution depends upon the antibody concentration in the original test sample. The antibody response to L. pneumophila may be both specific and nonspecific, since the patient may have antibodies to similar antigens from other Gram-negative bacteria. Optimum times for specimen collection appear to be within the first week of illness, or as soon as possible after the onset (acute specimen), and at least 3 weeks after the onset (convalescent specimen) (3). By the IFA method, a single result of >1/256 is considered presumptive evidence of legionella infection. Diagnostic titers have been reported to be absent in as many as 25% of patients (4), but the use of multiple Legionella species (5,6) as the antigen source and a polyvalent conjugate directed against IgG, IgM, and IgA (7) maximize the accuracy of serological procedures.Detection Range.Detection Range Qualitative: Positive, Negative and Cut-offSample: 10ulSpecificity: 95%Sensitivity: 95%Total Time: ~ 70 minShelf Life: 12-18monthsKit Components: Each kit contains the following components in sufficient quantities to perform the number of tests indicated. All reactive reagents contain 0.1% sodium azide as a preservative 1. Microtiter Plate2. Conjugate3. Calibrator4. Positive Control5. Negative Control6. TMB7. Wash Buffer, 10X Diluted wash buffer to 1X8. Sample Diluent9. Stop Solution Storage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Legionella, IgG, IgA, IgM, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Legionella, IgG, IgA, IgM, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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