- 中文名称
Lactoferrin, Human Plasma ELISA Kit, BioAssay
- 英文名字
- Lactoferrin, Human Plasma ELISA Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- L1021-90
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Human lactoferrin (LTF) is an 80kD glycoprotein which was first isolated from human milk. LTF is found in most body fluids and secretions, e.g., in the nose, genital tract, and tears.1 In the blood, LTF is secreted by neutrophils and its plasma concentration is positively related to the total pool of neutrophils and to the rate of neutrophil turnover.2 Because of its ability to strongly bind iron, LTF is considered to be bactericidal.3 In a number of cases of inflammation, LTF is released into the extracellular medium from secondary granules of neutrophilic leukocytes.4,5 Its extracellular concentration can therefore be used as an index of neutrophil activation, especially in blood samples containing anti-myeloperoxidase antibodies.Principle of the Procedure:This method is an Enzyme-linked immunosorbent assay (ELISA) for lactoferrin. Lactoferrin is captured by a monoclonal antibody (MAb) that is coated on wells of a sectional microplate. A second LTF-MAb labeled with biotin is added to the well and binds with the captured LTF forming a “sandwich.” A solution of streptavidin-peroxidase is then added. Streptavidin has a high affinity for biotin and once bound, its HRP (HRP) label is available for color development by addition of the substrate, o-phenylenediamine (OPD). This color development at 450nm is proportional to the quantity of LTF in the sample.Kit Format: 6x16wells (96 assays)Detection Limit: ~2ng/mlKit Components:L1021-90A: Sample Dilution Buffer: PBS, BSA, Tween-20 and 0.01% thimerosal, 3 x 25mlL1021-90B: LTF Standard: Approximately 300ng of purified LTF with BSA in lyophilized form, 1x1vialL1021-90C: Wash Buffer (20x), Tris-HCl buffer containing NaCl, Tween-20, and 0.01% thimerosal. 1x100ml L1021-90D: LTF Antibody, Concentrated solution of monoclonal antibody to LTF in phosphate buffer containing NaCl, BSA, glycerol, and 0.01% thimerosal. 1x75ulL1021-90E: Streptavidin-HRP, Concentrated solution of streptavidin-coupled HRP in Tris-HCl buffer containing BSA and 0.01% thimerosal. 1x75ulL1021-90F: Dilution Buffer, PBS, BSA, Tween-20, and 0.01% thimerosal, 3x8mlL1021-90G: OPD Diluting Buffer, Citrate buffer containing H2O2 and 0.01% thimerosal. 1x20mlL1021-90H: Stop Solution, 1M H2SO4. 1x20mlL1021-90J: OPD, Four tablets.L1021-90K: Microtiter plate, 1x96 wells Storage and Stability:Store *L1021-90B at 4°C; liquid at -20°C if not used within 3 days. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. L1021-90A and L1021-90F should be used within 5 days after opening of bottles.
- 产品特点
- 针对Lactoferrin, Human Plasma ELISA Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Lactoferrin, Human Plasma ELISA Kit, BioAssay产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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