Lactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormally high concentrations of lactate have been related to disease states such as diabetes and lactic acidosis, etc. L(+)‐Lactate is the major lactate stereoisomer formed in human intermediary metabolism and is present in blood. D(‐)‐Lactate is also present but only at about 1‐5% of the concentration of L(+)‐Lactate. In the Lactate Assay Kit, lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (lambda-max = 450nm). The kit detect L(+)‐Lactate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples. It detects 0.02mM-10mM lactate in various samples.Kit Components:Lactate Assay Buffer 1x25mlLactate Enzime Mix lyophilizedLactate Substrate Mix lyophilizedL(+)-Lactate Standard (100mM) 1x100ul.Storage and Stability:Store the kit at -20°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
