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细胞分析试剂盒
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中文名称

PicoProbeTM磷脂酸检测试剂盒(荧光法)

英文名字
PicoProbeTM Phosphatidic Acid Assay Kit (Fluorometric)
供应商
Biovision
产品货号
K748-100
产品报价
¥询价/100assays
产品说明书
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购买方式
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产品新闻
背景资料
待更新
产品描述
Phospholipids are amphiphilic molecules containing phosphate groups, and are both critical components of biological membranes and important signaling messengers in a number of biological pathways. Phosphatidic acid (PA) is the simplest phospholipid, consisting of a glycerol backbone with fatty acids bonded to carbon 1 and 2, and a phosphate group bonded to carbon 3. PA is a precursor for the synthesis of more complex phospholipids such as phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine that act as structural components of the plasma membrane. Phosphatidic acid itself is present in the plasma membrane lending the membrane a high curvature and a net negative charge. In addition, it is an important signaling lipid. It acts through a G-protein coupled receptor and is involved in growth regulation and mitosis. BioVision’s phosphatidic acid assay kit is a plate-based enzymatic assay for quantitation of PA in cells and tissues. The PA Converter hydrolyzes PA to form an intermediate which, in the presence of a developer and enzyme mix, converts a non-fluorescent probe to a fluorescent product (Ex/Em= 535/587 nm) that can be quantified. The Converter is specific to PA and does not hydrolyze other phospholipids (e.g. those with more complex head groups such as phosphatidylcholine or cardiolipin), allowing direct quantitation of PA. This kit can detect as low as 40 pmol PA per well
产品特点
Simple, fast, does not require lenghty incubation times
保存建议
-20
其他
BioVision的公司总部在美丽的旧金山湾地区,它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品,以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理,艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
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