- 中文名称
蛋白质羰基含量测定试剂盒（荧光法）
- 英文名字
- Protein Carbonyl Content Assay Kit (Fluorometric)
- 供应商
- Biovision
- 产品货号
- K563-100
- 产品报价
- ￥询价/100assays
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868.
- 产品新闻
- 背景资料
- 待更新
- 产品描述
- Protein carbonylation occurs when chronically elevated blood sugar levels create a microenvironment for sugars to covalently react with amino acids forming non-enzymatic adducts. Oxidative stress, the excessive production of ROS, can also give rise to the production of protein carbonyls. Over time, these two processess create Advanced Glycation End-products (AGEs) with proteins. The major functional outcome of protein-reactive AGEs is reduced enzymatic activity. Soluble AGEs can also bind and activate the receptor of advanced glycation end-products (RAGE). Receptor-dependent outcomes lead to tissue dysfunction, oxidative stress, and activation of monocytes, endothelial cells and mesangial cells. In Alzheimer’s Disease (AD) protein carbonyls are a marker of oxidative stress in the brain and are elevated in serum and plasma proportional to the cognitive severity. BioVision’s Protein Carbonyl Content Assay Kit uses Fluoroscein-5-Thiosemicarbazide (FTC), a fluorescent probe which covalently reacts with oxidized residues (i.e Cysteine, Lysine, Arginine, Histidine and Aspartic Acid) on proteins. The protein carbonyl content is determined by the generation of a stable fluorometric signal (Ex/Em 485/535 nm) and compared to the protein concentration determined in the BCA Assay to quantitate nmoles of carbonyl/mg protein. The kit is simple, requires no harsh chemicals, can quantitate carbonyls in serum or plasma and produces more reliable and reproducible results than the comparable colorimetric assays. It can detect carbonyl groups in samples with protein concentrations as low as 1 mg/ml!
- 产品特点
- • Simple procedure; validated with human serum samples containing pmol of carbonylated protein
- 保存建议
- -20
- 其他
- BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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