- 中文名称
EZClick™O-GalNAc修饰糖蛋白测定试剂盒（FACS /显微镜，绿色荧光）
- 英文名字
- EZClick™ O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
- 供应商
- Biovision
- 产品货号
- K560-100
- 产品报价
- ￥询价/100assays
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868.
- 产品新闻
- 背景资料
- 待更新
- 产品描述
- Glycans are vital components of glycoproteins, glycolipids, and proteoglycans in all domains of life. Glycoproteins are grouped by the type of carbohydrate and amino acid linkage site. N-linked glycosylation is a modification of asparagine, whereas O-linked glycosylation occurs through the hydroxyl group of serine and threonine residues. Glycosylation occurs co- or post-translationally on >50% of eukaryotic proteins resulting in membrane-associated, intracellular, or secreted glycoproteins that are crucial in cellular processes, protein bioactivity and metabolic turnover. Attachment of O-linked mucin-type glycans is a common post-translational modification initiated by the addition of N-acetyl-galactosamine (GalNAc) to a Ser or Thr residue of newly assembled protein. Resulting GalNAc-(Ser/Thr) structure (Tn-antigen) can be further modified by the addition of either a sialic acid residue (sialyl-Tn), GlcNAc to form GlcNAc-GalNAc (core 3), or a galactose residue to form the GalGalNAc (core 1) structures respectively. The core 3 and 1structures are then further extended to form long and branched complex O-glycans. The core 1 (TF or T antigen) acts like oncofetal antigen, overexpressed in cancerous and precancerous conditions due to the Golgi apparatus disorder. GalNAc linked to the first mannose of glycosylphosphatidylinositol (GPI) core has been previously reported to be heterogeneously present on mammalian GPI-anchored proteins. Thus BioVision offers EZClickTM O-GalNAc Modified Glycoprotein Assay Kit, a highly specific, simple and robust method for labeling and detection of O-GalNAc-glycosylated proteins within cells. We use a modified galactosamine precursor that is fed directly into the cells, processed by the GalNAc salvage pathway to form the intermediate uridine diphospho (UDP)-GalNAz, which is recognized by GalNAc transferases in the Golgi and incorporated into the protein. Followed by click reaction with alkyne-containing dye, this system offers a powerful method for imaging the localization, trafficking, and dynamics of glycans, or detection by FACS for quantitative studies. Labeled Glycoproteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-alkyne or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format
- 产品特点
- Simple, fast, does not require lengthy incubation times
- 保存建议
- -20
- 其他
- BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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