- 中文名称
可溶性胶原蛋白检测试剂盒（荧光法）
- 英文名字
- Soluble Collagen Assay Kit (Fluorometric)
- 供应商
- Biovision
- 产品货号
- K532-100
- 产品报价
- ￥询价/100assays
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868.
- 产品新闻
- 背景资料
- 待更新
- 产品描述
- Collagen is the most abundant protein in mammals, accounting for nearly 30% of body protein content. Collagen is an extracellular scaffolding protein found in skin, tendons, bone, cartilage, ligaments and muscles, amongst other tissues. There are more than twenty different characterized types of collagen, but the fibrilous types I, II and III comprise more than 90% of the total collagen in mammals. Collagen has a triple helical structure, consisting of three ‘preprocollagen’ strands with a repeating glycine- and proline-rich tripeptide sequence (Gly-Pro-X) and small end-cap domains called registration peptides. Nascent preprocollagen strands undergo enzymatic post-translational hydroxylation at proline and lysine residues prior to secretion to the extracellular milieu via the golgi apparatus. Once outside the cell, the dangling registration peptides are cleaved off, forming tropocollagen, the monomeric precursor to mature collagen fibers. While mature collagen exists as an insoluble web of extensively crosslinked fibrils, tropocollagen is soluble in dilute acidic solutions. Many diseases are believed to affect collagen synthesis, fibrilization and turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy. BioVision’s Soluble Collagen Assay Kit allows for quantification of newly synthesized (acid-soluble) collagen levels in tissues and cultured cell medium. The assay involves extraction of soluble collagen in acetic acid, followed by enzymatic degradation of collagen into glycine-rich oligopeptides, which are quantified using a fluorogenic reagent and developer solution that selectively react with the N-terminal glycine fragments to form a stable fluorescent complex (Ex/Em = 376/468 nm). The assay is more sensitive and selective than dye-binding (Picrosirius Red) soluble collagen assays, is simple to perform and has a linear range from 0.05 – 2 μg collagen per well (2.5 μg/ml to 100 μg/ml in a 20 μl sample volume)
- 产品特点
- • Simple, fast and convenient assay
• Can detect as low as 0.05 µg of soluble collagen in a variety of samples
• Simple procedure; takes less than ~60 minutes
• Increased sensitivity (Fluorometric Detection)
• PCA-free
- 保存建议
- -20
- 其他
- BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
