中文名称

KBBC培养基

英文名字

KBBC Medium

供应商

Duchefa Biochemie B.V.

产品货号

K5120.1000

产品报价

￥询价/1kg

产品说明书

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购买方式

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产品新闻

背景资料

Pseudomonas syringae pv. syringae (Pss) is the causal organism of bacterial brown spot of beans. This bacterium is seed borne and therefore its detection on seeds is important. KBBC medium is a rather selective medium to detect Pss on seeds of beans. This medium is based on King’s B Medium (K5165), however in KBBC Medium boric acid (1.5 g/liter), cephalexin and nystatin are added. Nystatin is used to control fungi. As an alternative, cycloheximide, a more potent fungicide, can be used. KBBC is much more selective than MSP (M5167) and in general the recovery of Pss is smaller on KBBC than on MSP. Pspha, unlike Pss, will not grow on KBBC. Therefore, the chance of detection of Pss is higher when both complementary media are used.Detection of Pss is performed by the dilution plating of bacterial extract on KBBC and MSP. Then Pss-suspected isolates are transferred to KB medium. Finally, the identification of suspected colonies can be performed by a pathogenicity assay or PCR.Colonies of Pss on KBBC are 3-4 mm in diameter, flat, circular, translucent, creamy white and show blue fluorescence under UV light. This medium can also be used for the detection of seed borne Ps porri, Ps pisi and Ps tomato on seed of resp. leek, pea and tomato.

产品描述

Pseudomonas syringae pv. syringae (Pss) is the causal organism of bacterial brown spot of beans. This bacterium is seed borne and therefore its detection on seeds is important. KBBC medium is a rather selective medium to detect Pss on seeds of beans. This medium is based on King’s B Medium (K5165), however in KBBC Medium boric acid (1.5 g/liter), cephalexin and nystatin are added. Nystatin is used to control fungi. As an alternative, cycloheximide, a more potent fungicide, can be used. KBBC is much more selective than MSP (M5167) and in general the recovery of Pss is smaller on KBBC than on MSP. Pspha, unlike Pss, will not grow on KBBC. Therefore, the chance of detection of Pss is higher when both complementary media are used.Detection of Pss is performed by the dilution plating of bacterial extract on KBBC and MSP. Then Pss-suspected isolates are transferred to KB medium. Finally, the identification of suspected colonies can be performed by a pathogenicity assay or PCR.Colonies of Pss on KBBC are 3-4 mm in diameter, flat, circular, translucent, creamy white and show blue fluorescence under UV light. This medium can also be used for the detection of seed borne Ps porri, Ps pisi and Ps tomato on seed of resp. leek, pea and tomato.

产品形式

固体

保存建议

建议收到产品后，按照说明书保存建议存放。

其他

Duchefa Biochemie B.V.于1989年成立于荷兰的哈勒姆市。专门研究和供应植物生物技术中使用的培养基和化学品,并且有非常严格的安全要求和质量标准。用于植物生物技术的产品总数已达400多种,主要包括用于植物分子生物学和种子健康测试的培养基,生长调节剂,抗生素,胶凝剂,底物和化学品。更有侧重于植物病理学用途的产品,包括20多种用于测试各种农作物种子上病原体的培养基。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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