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细胞分析试剂盒
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中文名称

总多胺测定试剂盒(荧光法)

英文名字
Total Polyamine Assay Kit (Fluorometric)
供应商
Biovision
产品货号
K475-100
产品报价
¥询价/100assays
产品说明书
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产品新闻
背景资料
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产品描述
Polyamines are small organic molecules bearing two or more primary amine moieties. Biogenic polyamines such as spermidine, putrescine and spermine function as transcellular signaling molecules and are involved in diverse biological processes. Although small amounts of polyamines are synthesized in cells, larger quantities are often encountered as a result of putrefaction and decay, as they are the direct product of decarboxylation of amino acids such as methionine, lysine and arginine. The enzyme ornithine decarboxylase generates the polyamine putrescine, which is not only responsible for repulsive odor but is also implicated in cancer. Intracellular polyamines readily bind DNA and are critical to preventing oxidative DNA damage and directing DNA double-strand break repair pathways. Polyamine levels decline with age and dietary supplementation of the polyamine spermidine has recently been shown to reduce age-related oxidative stress and extend lifespan in mouse models of aging. BioVision’s Total Polyamine Detection Kit enables the rapid determination of polyamine concentration in biological samples. A selective enzyme mix acts on polyamines, generating hydrogen peroxide that is then reacted with a fluorometric probe (Ex/Em = 535/587 nm) to yield a signal proportional to the amount of polyamine present. The kit includes a proprietary Sample Clean-Up reagent for pre-treating samples in order to eliminate common metabolites found in biological samples that may interfere with the assay or increase sample background. The assay is rapid, simple, and high throughput compatible, and can detect polyamine concentrations as low as 0.1 µM in tissue lysates and other samples such as saliva.
产品特点
• Simple, highly sensitive, high-throughput compatible
保存建议
-20
其他
BioVision的公司总部在美丽的旧金山湾地区,它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品,以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理,艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
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