- 中文名称
EZClick™全球蛋白质合成测定试剂盒
- 英文名字
- EZClick™ Global Protein Synthesis Assay Kit
- 供应商
- Biovision
- 产品货号
- K467-100
- 产品报价
- ￥询价/100assays
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868.
- 产品新闻
- 背景资料
- 待更新
- 产品描述
- Protein synthesis is a tightly regulated process and many critical controls in gene expression occur at the level of translation to ensure that production of specific cellular proteins is quickly turned on/off under specific conditions (heat shock, starvation, etc). It is essential in cell growth, proliferation, signaling, differentiation or death; therefore, the identity and amount of the synthesized proteins are critical in determining the physiological state of the cell. Methods of detection and characterization of nascent proteins, or changes in spatial and temporal protein expression/degradation patterns during disease, drug treatments or environmental changes are important for assessment of cytotoxicity. Biovision’s EZClick™ Global Protein Synthesis Assay Kit utilizes a novel chemical method based on alkyne analog of puromycin, O-Propargyl-puromycin (OP-puro). OP-puro stops translation by forming covalent conjugates with nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide (Ex/Em= 494/521 nm). OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture. We provide sufficient materials for 100 simple and specific assays to detect nascent proteins synthesized under various physiological conditions in the real-time, and Cycloheximide, an inhibitor of protein synthesis that serves as a control
- 产品特点
- Simple, fast, does not require lengthy incubation times
- 保存建议
- -20
- 其他
- BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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